Mass Spectrometry training and equipment

The hands-on training courses have been regularly over-subscribed and attended by researchers within all themes (see Chart 1 below).  Attendees have been at all levels of training (see Chart 2 below), but are predominately DPhil students and post-docs. In addition, courses have been well-received, with feedback forms showing that participates routinely ranked the courses as ‘excellent’ or ‘very good’ (from 10 of 10 feedback forms collected over all 4 courses).

Since inception of the infrastructure award, CRE researchers have run 298 samples at the Advanced Proteomics Core Facility. All samples have received a 20% discount and researchers have benefited from free sample preparation and experiment analysis, together equating to a total estimated savings of £17,000.

In addition, the award has provided training in proteomics methods which has allowed for new collaborations between early career researchers (e.g. funding of two interdisciplinary grants with CRE investigators as named collaborators specifically to perform mass spectrometry analysis) and aided in grant applications and/or publications from a number of PIs.

Publications linked to this award:

1. Bailey, J., Shaw, A., Fischer, R., Ryan, B., Kessler, B., McCullagh, J., Wade-Martins, R., Channon, K. and Crabtree, M. (2017). A novel role for endothelial tetrahydrobiopterin in mitochondrial redox balance. Free Radical Biology and Medicine, 104, pp.214-225.
2. Bonnici, J., Tumber, A., Kawamura, A., & Schofield, C. J. (2017). Inhibitors of both the N-methyl lysyl- and arginyl- demethylase activities of the JmjC oxygenases. Philosophical Transactions B: Biological Sciences.
3. Eaton, J., Alenazi, Y., Singh, K., Davies, G., Geis-Asteggiante, L., Kessler, B., Robinson, C., Kawamura, A. and Bhattacharya, S. (2018). The N-terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins. Journal of Biological Chemistry, 293(16), pp.6134-6146.