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Mala Rohling

Senior Research Technician

Oxford

DEPARTMENTS

University of Oxford: Department of Physiology, Anatomy & Genetics

University of Oxford: Cardiovascular Medicine - BMRU

 

RESEARCH INTERESTS

I am interested in new techniques to study developmental biology, for both basic scientific research and as a potential tool for regenerative medicine. This may involve animal models as well as in vitro cell and tissue culture methods.

My primary role for the BHF CRM is to perform a series of myocardial infarctions in mice (adult and neonate, wild type and various genetic models) to provide a platform to test various therapeutic regenerative strategies. I am also responsible for training other colleagues and students in these surgical techniques.

 

PROJECTS

-          Cardiac lymphatics are heterogeneous in origin and response to injury

-          BRG1-SWI/SNF-dependent foetal gene reprogramming dynamically regulates epicardial progenitor activity following cardiac injury

-          Myocardial infarction stimulates new lymphatic vessel formation by lymphangiogenesis

-          Diminishing the pro-inflammatory immune response alters cardiac healing after myocardial infarction

-          Does loss of Hif2α expression result in a decrease in epicardial activation post MI?

-          Neovascularisation of the Heart post-MI: A Role for Endocardial Trabeculation?

-          Tomo-seq: A new method to determine genetic changes in different areas within an injured heart.

-          MRI analysis of neonate P1 and P7 MI heart function from baseline (Day 0) to Day 21.

-          Endothelial cell-derived extracellular vesicles promote splenic monocyte mobilisation in acute myocardial infarction.’

 

KEYWORDS

Mouse surgical models, myocardial infarction, regenerative medicine, cardiac development, growth and regeneration.

PUBLICATIONS

Cardiac lymphatics are heterogeneous in origin and respond to injury. Klotz L, Norman S, Vieira JM, Masters M, Rohling M, Dubé KN, Bollini S, Matsuzaki F, Carr CA, Riley PR. Nature. 2015 Jun 4;522(7554):62-7.

Facilitation by intracellular carbonic anhydrase of Na+ -HCO3- co-transport but not Na+ / H+ exchange activity in the mammalian ventricular myocyte. Villafuerte FC, Swietach P, Youm JB, Ford K, Cardenas R, Supuran CT, Cobden PM, Rohling M, Vaughan-Jones RD. J Physiol. 2014 Mar 1;592(5):991-1007. doi: 10.1113/jphysiol.2013.265439.

 

ABSTRACTS & CONFERENCE PRESENTATIONS

Origins of Cardiac Lymphatics. Klotz L, Norman S, Vieira JM, Masters M, Rohling M, Dubé KN, Bollini S, Matsuzaki F, Carr CA, Riley PR. British Society for Cell & Gene Therapy/British Society for Cardiovascular Research Joint Conference, Glasgow 2015

High-resolution MR Imaging of left-ventricular function in the newborn mice. Mahon L Maguire, Mala Rohling, Megan Masters, Debra McAndrew, Paul Riley, Jurgen E Schneider. ISMRM International Society for Magnetic Resonance in Medicine 2016 Annual Meeting & Exhibition, Singapore

 

RESEARCH PROJECT LEADERS

Professor Paul Riley, Department of Physiology, Anatomy & Genetics, University of Oxford

-          Dr. Joaquim Vieira

-          Dr. Damien Barnette

-          Dr. Thomas Cahill

-          Dr. Sophie Norman

-          Ellie Price, DPhil Student

Dr. Nicola Smart, Department of Physiology, Anatomy & Genetics, University of Oxford

-          Tonia Thomas, DPhil Student

Dr. Sarah De Val, Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford

Professor Jurgen Schneider, Cardiovascular Medicine, University of Oxford

Dr. Robin Choudhary, Cardiovascular Medicine, University of Oxford

Dr. Jyoti Patel, Cardiovascular Medicine, University of Oxford

Dr. Marco Meloni, Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre University of Glasgow

Professor Jeroen Bakkers, Hubrecht Institute, Utrecht University, Netherlands

-          Fabian Kruse, PhD Student