Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.
Journal article
2000-02-15T00:00:00+00:00
28
Animals, Animals, Genetically Modified, Fertilization, Gene Transfer Techniques, Green Fluorescent Proteins, Luminescent Proteins, Male, Ovum, Sperm Injections, Intracytoplasmic, Spermatozoa, Xenopus, Xenopus laevis