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During mouse embryonic development, the T-box transcription factor Eomes/Tbr2 is expressed in highly dynamic patterns in various progenitor cell types. Those include the undifferentiated cells of the trophectoderm, ingressing nascent mesoderm at the primitive streak, and intermediate progenitor cells of the developing cerebral cortex. We generated an Eomes(GFP)- targeted allele to follow the highly dynamic patterns of Eomes expression and to allow for the identification of novel expression domains. We show that our novel allele recapitulates endogenous gene expression at known sites of expression and confirm our results by anti-Eomes immunofluorescent staining. Using this novel allele we were able to identify previously undocumented domains of Eomes expression within the visceral endoderm and at various locations in the developing and adult mouse brain.

Original publication

DOI

10.1002/dvg.20562

Type

Journal article

Journal

Genesis

Publication Date

11/2009

Volume

47

Pages

775 - 781

Keywords

Alleles, Animals, Base Sequence, DNA Primers, Genes, Reporter, Green Fluorescent Proteins, In Situ Hybridization, Mice, T-Box Domain Proteins