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We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

Original publication

DOI

10.1038/nmeth.2841

Type

Journal article

Journal

Nat Methods

Publication Date

04/2014

Volume

11

Pages

403 - 406

Keywords

Animals, Mass Spectrometry, Models, Molecular, Protein Conformation, Proteins