Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

Original publication

DOI

10.1093/nar/28.4.e12

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

15/02/2000

Volume

28

Keywords

Animals, Animals, Genetically Modified, Fertilization, Gene Transfer Techniques, Green Fluorescent Proteins, Luminescent Proteins, Male, Ovum, Sperm Injections, Intracytoplasmic, Spermatozoa, Xenopus, Xenopus laevis