Training opportunities in mass spectroscopy-based ‘omics techniques
Overview:
The ‘Omics Training Scheme was established by the BHF Centre for Research Excellence, in collaboration with the Advanced Proteomics Facility at the Target Discovery Institute (TDI), with a view to increase awareness, accessibility and training in mass spectroscopy-based ‘omics research across the University.
The scheme is organised and chaired by Dr Jillian Simon, Dr Mark Crabtree and Dr Roman Fischer and offers a number of training opportunities, including: seminars on the application of mass spectroscopy to ‘omics research; hands-on training in various ‘omics workflows; and advanced training in state-of-the-art technology and novel workflows, including the opportunity for DPhil students to undertake a lab rotation at the TDI. In addition to the training programme, the scheme offers CRE members direct access to ‘omics infrastructure within the Advanced Proteomics Facility at a strongly reduced cost.
For further information on training and discounts, please contact either Jillian Simon or Mark Crabtree.
Seminars and Courses:
Members of the BHF CRE are invited to participate in seminars and hands-on workshops covering an array of mass spectroscopy workflows. The programme of courses varies from term to term, with some courses running more frequently than others according to demand. An up-to-date schedule of courses and seminars can be found below. Courses are free of charge, but registration is required.
* SEMINARS:
No seminars currently offered.
Past seminars: Mass Spectroscopy-based Approaches to ‘Omics Research (October 2015)
* HANDS-ON COURSES:
No courses currently offered.
Past course: Basics of Protein Sample Processing & Analysis (March 2016)
In this 3-day training workshop participants will be provided an introduction on how to use proteomics techniques to address a number of biological questions and learn how to process and analyse samples using mass spectroscopy. The course consists of 2 theoretical and 3 practical modules and is aimed at those with little or no background in proteomic workflows.
Programme:
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Monday, 14th March |
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10:00 – 12:00 |
Introduction to mass spectroscopy-based proteomics: How it works, experimental design and options for quantitative analysis |
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13:00 – 17:00 |
Hands-on Sample Prep*: in-solution or in-gel tryptic digest of your own sample (day 1) |
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Tuesday, 15th March |
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10:00 – 12:00 |
Hands-on Sample Prep*: in-solution or in-gel tryptic digest of your own sample (day 2) |
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13:00 – 16:00 |
Fundamentals of Data Analysis: Using MASCOT for database searching, label-free quantitation and free alternatives for data analysis |
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Thursday, 17th March |
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10:00 – 12:00 |
Analyse Your Own Data: database searching and interpretation of your MS results |
* Participants are asked to bring their own sample for use in the practical modules. This can be either a protein or protein mixture in solutions, or an SDS-PAGE gel band. Alternatively, we will provide a total cell lysate for processing.
* ADVANCED TRAINING:
For details on advanced training opportunities, including arrangement of DPhil lab rotations (limit of 2 students/year) please contact Roman Fischer.
Contact Jillian Simon (jillian.simon@cardiov.ox.ac.uk)
Advanced Proteomics Facility, Target Discovery Institute