Figure 4 from Oncogenic Cell Tagging and Single-Cell Transcriptomics Reveal Cell Type–Specific and Time-Resolved Responses to Vhl Inactivation in the Kidney
Kurlekar S., Lima JDCC., Li R., Lombardi O., Masson N., Barros AB., Pontecorvi V., Mole DR., Pugh CW., Adam J., Ratcliffe PJ.
Renal cortex, but not the papilla, is permissive to long-term survival of Vhl-null cells. A, Representative tdTomato IHC counterstained with hematoxylin in different renal anatomical regions of Control or KO mice harvested at the early or late time points. Scale bar, 100 μm. Magnification, ×20. B, Proportion of cells that are tdTomato-positive in different regions of the kidney as quantified by tdTomato IHC in kidneys from Control or KO mice harvested at different intervals after recombination. n = 7F, 16M for all regions for KO; n = 9F, 15M; 9F, 15M; 9F, 14M; 8F, 14M for cortex, outer medulla, inner medulla, and papilla, respectively, for Control. Line denotes linear regression. Significance testing performed for slope and intercept of linear regression by t test.