Effect of mutation Arg91Gly on the thermal stability of β-tropomyosin
Nevzorov IA., Redwood CS., Levitsky DI.
The mutation Arg91Gly (R91G) in β-tropomyosin (β-TM) is known to cause distal arthrogryposis, a severe congenital disorder of muscle tissues. The influence of this mutation in β-TM on its structure and thermal denaturation was demonstrated. It was shown by the differential scanning calorimetry and circular dichroism that this point mutation dramatically decreased the thermal stability of the significant part of the β-TM (about a half of the molecule). This part of the β-TM molecule carrying R91G mutation unfolds at ∼ 28°C, i.e., at a much lower temperature than the other part of the molecule, which melts at ∼40°C. The data of the differential scanning calorimetry were compared with the results of temperature dependence of pyrene eximer fluorescence, which decreased upon the dissociation of two β-TM chains in the region of pyrene-labeled Cys-36. This comparison allowed one to conclude that this thermal transition reflected the thermal unfolding of the whole N-terminal part of β-TM. Interestingly, the destabilizing effect of Arg91Gly mutation spread for a rather long distance along the tropomyosin coiled-coil indicating a high cooperativity of the thermal denaturation within this part of β-TM. © 2008 Pleiades Publishing, Ltd.