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We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent. © 2013 Nature America, Inc.

Original publication

DOI

10.1038/nmeth.2691

Type

Journal article

Journal

Nature Methods

Publication Date

01/12/2013

Volume

10

Pages

1206 - 1208