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The ability to maintain intact ribosomes in the mass spectrometer has enabled research into their changes in conformation and interactions. In the mass spectrometer, it is possible to induce dissociation of proteins from the intact ribosome and, in conjunction with atomic structures, to understand the factors governing their release. We have applied this knowledge to interpret the structural basis for release of proteins from ribosomes for which no high resolution structures are available, such as complexes with elongation factor G and ribosomes from yeast. We also describe how improvements in technology and understanding have widened the scope of our research and lead to dramatic improvements in quality and information available from spectra of intact ribosomes.

Original publication

DOI

10.1016/j.febslet.2004.12.003

Type

Journal article

Journal

FEBS Lett

Publication Date

07/02/2005

Volume

579

Pages

943 - 947

Keywords

Ions, RNA, Ribosomal, Ribosomal Proteins, Ribosomes, Spectrometry, Mass, Electrospray Ionization