Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

Type

Journal article

Journal

J Mol Biol

Publication Date

16/01/2004

Volume

335

Pages

685 - 692

Keywords

Binding Sites, DNA-Directed RNA Polymerases, Electrophoresis, Polyacrylamide Gel, Escherichia coli Proteins, Molecular Weight, Mutagenesis, Site-Directed, Protein Binding, Repressor Proteins, Sigma Factor, Spectrometry, Mass, Electrospray Ionization