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Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 microg (median 54.3 microg; mean 82.2 microg +/- SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 microg) than those from women (median 44.2 microg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.

Original publication

DOI

10.1007/s10654-009-9388-x

Type

Journal article

Journal

Eur J Epidemiol

Publication Date

2009

Volume

24

Pages

677 - 682

Keywords

Age Factors, Aged, Antihypertensive Agents, Case-Control Studies, DNA, Female, Humans, Male, Middle Aged, Mouth Mucosa, Sex Factors, Specimen Handling