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Genetic and chemical analysis of cardiomyocyte energy and nutrient homeostasis

  • 1 January 2011 to 31 December 2011
  • Awards: Pump-priming Awards

The aim of the project, as part of a new program of target and therapeutic discovery was to develop biosensors to discover new therapeutics using high-throughput screens.  While we were unable to develop an energy biosensor with sufficient Z'factor suitable for high-throughput screens, we were able to develop using the biosensor technology a eNOS dimerisation split renilla lucifrease biosensor, that has since been applied to studies of eNOS biology.

Publication linked to this award:

  1. Benson, M. A., Batchelor, H., Chuaiphichai, S., Bailey, J., Zhu, H., Stuehr, D. J., Bhattacharya, S., Channon, K. M., and Crabtree, M. J. (2013) A pivotal role for tryptophan 447 in enzymatic coupling of human endothelial nitric oxide synthase (eNOS): effects on tetrahydrobiopterin-dependent catalysis and eNOS dimerization. J Biol Chem 288, 29836-29845