PENTRA C400 is a chemistry analyser that performs diagnostic tests on whole blood, serum, plasma and urine samples using spectrophotometry and potentiometry. We have used the PENTRA to analyse iron, ferritin and transferrin levels in serum samples from humans as well as mice, giving us an overall body iron status. Measurement of other proteins are also available.

Samples are typically diluted 1 in 25 and require a volume of 200 µl. The system allows loading of 60 test samples at one time with each specific protein test requiring calibrators and controls

Sherrington Building, Level 1

Contact: Syeeda Nashitha Kabir

Open to BHF-CRE researchers on a collaborative basis without charge, but researchers must purchase relevant calibrators.

Loick P, Mohammad G, Cassimjee I, Chandrashekar A, Lapolla P, Carrington A, Handa A, Lee R, Lakhal-Littleton S. (2023) Protective Role For Smooth Muscle Cell Hepcidin In Abdominal Aortic Aneurysm. Arterioscler Thromb Vasc Biol. 2023 May;43(5):713-725.

• Mohammad G, Matakidou A, Robbins PA, Lakhal-Littleton S  (2021) The kidney hepcidin/ferroportin axis controls iron reabsorption and determines the magnitude of kidney and systemic iron overload. Kidney Int. S0085-2538(21)00490-7.

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• Kammerer L*, Mohammad G*, Wolna M*, Robbins P, Lakhal-Littleton S (2020) Fetal liver hepcidin supports acquisition of iron stores in utero. Blood 136 (13): 1549–1557.