Fast Protein Liquid Chromatography (FPLC)
- Features: 3 buffer channels, direct sample loading including bubble sensor, UV and conductivity meter, fraction collector for 12 ml tubes (0.1 - 5 ml).
- Columns available (with permission) Anion (Q-FF) and cation (S-FF) exchange, Nickle NTA His trap, HIC, gel filtration column (sepadex 200), Hydroxyappatate.
- Other compatible columns: Protein A/G affinity columns for monoclonal antibody purification.
|Output trace showing the loading and elution of recombinantly expressed protein from BL21 E.coli lysate using a HisTrap Nickle NTA resin column. UV trace shows high levels of unbound waste protein removed in the broad peak (left), and subsequent purified protein elution peak (right) and corresponding fractions collected beneath|
SDS PAGE of purified fractions 1-12 the waste flowthrough (ft) for recombinant cardiac troponin I expressed in BL21 E.coli.
No charge. May need to provide filtered and degassed buffers, columns are disposable in some cases so will need to be purchased separately.
Sparrow AJ et al: Measurement of Myofilament-Localized Calcium Dynamics in Adult Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy Mutations. Circ Res. 2019 Apr 12;124(8):1228-1239.
Robinson P et al: Dilated and hypertrophic cardiomyopathy mutations in troponin and alpha-tropomyosin have opposing effects on the calcium affinity of cardiac thin filaments. Circ Res. 2007 Dec 7;101(12):1266-73.