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Upright Leica SP5 Confocal with 4 Objectives

  • Three lasers: green, red and far red
  • LED for imaging nuclei
  • Four objectives: 10x, 20x, 40x and 63x
  • Immunofluorescence imaging of fixed samples (tissue sections, whole cells, subcellular compartments)
  • A 20x water dipping lens for live imaging is also available

Leica SP5 confocal with 4 objectives.jpg

Availability

No charges

Training required

Location

Level 5, West Wing

John Radcliffe Hospital

Contact Paul Robinson

Example Data

Leica Confocal Data 1

Human induced pluripotent stem cell derived cardiomyocytes stained with alpha-actinin sarcomeric marker in green. Use of 63x oil objective.

Leica Confocal Data 2

Cryosections of mouse tissue stained for a sarcomeric marker (red), and an intercalated disk marker in blue. The protein of interest (filamin C, in green), localises to the intercalated disks. Use of 20x objective.

Images provided by Dr Katja Gehmlich