|Fluorescent 1 and 2 cell embryos|
|A single ES cell colony expressing Green Fluorescent Protein growing on a fibroblast feeder layer|
|Microinjection of embryonic stem cells into a blastocyst|
Dr Ben Davies
Transgenic Core Head
Genetically modified models represent one of the most powerful methods of functional gene analysis in vivo. Furthermore, the ability to introduce specific mutations into the genome enables models of human disease to be generated, facilitating insights into the pathophysiology of disease and providing a model with which therapeutic strategies and diagnostic tools can be optimized.
Our group provides groups within Oxford University access to transgenic technologies both on a fee-for-service type arrangement and on a collaborative basis. Technologies offered include embryo microinjection, embryonic stem cell transfection, Knock-out/-in construct design and in vivo shRNA mediated gene Knock-down. In addition, embryo rederivation and cryoconservation services are offered to facilitate the management, transfer and security of genetically modified strains.
The research activity of the group is focused on the development of novel methodologies for the generation of genetically modified models. The aims being to improve the reliability of the technology and to reduce the animal cost of research involving genetically modified models.
Altering the binding properties of PRDM9 partially restores fertility across the species boundary
DAVIES B., (2021), Molecular Biology and Evolution
When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping.
Sailer S. et al, (2021), Cell Biosci, 11
Phenotype of a transient neonatal diabetes point mutation (SUR1-R1183W) in mice
Sachse G. et al, (2021), Wellcome Open Research, 5, 15 - 15
Base editing and prime editing in laboratory animals
DAVIES B. and Caso F., (2021), Laboratory Animals
Functional analysis of a gene-edited mouse to gain insights into the disease mechanisms of a titin missense variant
JIANG H. et al, (2021), Basic Research in Cardiology