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Hirst et al claim that proteins ejected directly from mitochondrial membranes in our study are degraded, are incorrectly assigned, lack lipids, and show discrepancies with "native states" mostly obtained in detergent micelles. Here, we add further evidence in full support of our assignments and show that all complexes are either ejected intact or in known intermediate states, with core subunit interactions maintained. None are degraded or rearranged.

Original publication

DOI

10.1126/science.aax3102

Type

Journal article

Journal

Science (New York, N.Y.)

Publication Date

11/2019

Volume

366

Addresses

Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3QZ, UK. dror.chorev@chem.ox.ac.uk carol.robinson@chem.ox.ac.uk.

Keywords

Lipids, Membrane Proteins, Detergents, Mitochondrial Membranes, Mass Spectrometry