Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.

Original publication




Journal article



Publication Date





1196 - 1199


Amino Acid Motifs, Animals, Cell Cycle Proteins, Cell Line, Tumor, Centrioles, Centrosome, Chlamydomonas reinhardtii, Chromosomal Proteins, Non-Histone, Crystallography, X-Ray, Flagella, Humans, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Mutant Proteins, Point Mutation, Protein Multimerization, Protein Structure, Quaternary, Protein Structure, Tertiary, Recombinant Proteins, Zebrafish, Zebrafish Proteins