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Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations.

Original publication

DOI

10.1016/j.xpro.2021.100359

Type

Journal article

Journal

STAR Protocols

Publisher

Cell Press

Publication Date

26/02/2021

Volume

2