Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo.
Marshall KM., Wojciechowski JP., Jayawarna V., Hasan A., Echalier C., Øvrebø Ø., Yang T., Zhou K., Kanczler JM., Mata A., Salmeron-Sanchez M., Stevens MM., Oreffo ROC.
Bone tissue engineering aims to harness materials to develop functional bone tissue to heal 'critical-sized' bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of the coatings, three bioactive coatings were examined, i) elastin-like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenetic protein-2 (BMP-2) applied sequentially (PEA/FN/BMP-2) and iii) both ELP and PEA/FN/BMP-2 coatings applied concurrently. The scaffold material was robust and showed biodegradability. The coatings demonstrated a significant (p