Evidence that the catenane form of CS2 hydrolase is not an artefact.
van Eldijk MB., van Leeuwen I., Mikhailov VA., Neijenhuis L., Harhangi HR., van Hest JCM., Jetten MSM., Op den Camp HJM., Robinson CV., Mecinović J.
CS2 hydrolase, a zinc-dependent enzyme that converts carbon disulfide to carbon dioxide and hydrogen sulfide, exists as a mixture of octameric ring and hexadecameric catenane forms in solution. A combination of size exclusion chromatography, multi-angle laser light scattering, and mass spectrometric analyses revealed that the unusual catenane structure is not an artefact, but a naturally occurring structure.