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We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

Original publication

DOI

10.1038/nmeth.2691

Type

Journal article

Journal

Nat Methods

Publication Date

12/2013

Volume

10

Pages

1206 - 1208

Keywords

Detergents, Diacylglycerol Kinase, Diffusion, Escherichia coli, Escherichia coli Proteins, Green Fluorescent Proteins, Halobacteriaceae, Lipids, Magnetic Resonance Spectroscopy, Mass Spectrometry, Membrane Proteins, Micelles, Microscopy, Electron, Transmission, Monosaccharide Transport Proteins, Nanoparticles, Plasmids, Sensory Rhodopsins, Spectrometry, Mass, Electrospray Ionization, Symporters