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Mass spectrometry methods are being developed that enable detection of protein interactions with nucleic acids. By mass measuring complexes a direct determination of the stoichiometry of protein-nucleic acid interactions is revealed. For more complex assemblies, using a different approach it is possible to gain information about subcomplexes and even the spatial arrangement of proteins in macromolecular machines. To illustrate these different approaches we review progress and problems encountered in evaluating complexes from bimolecular interactions through to macromolecular machines such as ribosomes.

Original publication

DOI

10.1074/jbc.R300037200

Type

Journal article

Journal

J Biol Chem

Publication Date

11/06/2004

Volume

279

Pages

24907 - 24910

Keywords

Mass Spectrometry, Nucleic Acids, Oligonucleotides, Proteins, RNA