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The dynamics of protein complexes are crucial for their function yet are challenging to study. Here, we present a nanoelectrospray (nESI) mass spectrometry (MS) approach capable of simultaneously providing structural and dynamical information for protein complexes. We investigate the properties of two small heat shock proteins (sHSPs) and find that these proteins exist as dodecamers composed of dimeric building blocks. Moreover, we show that these proteins exchange dimers on the timescale of minutes, with the rate of exchange being strongly temperature dependent. Because these proteins are expressed in the same cellular compartment, we anticipate that this dynamical behavior is crucial to their function in vivo. Furthermore, we propose that the approach used here is applicable to a range of nonequilibrium systems and is capable of providing both structural and dynamical information necessary for functional genomics.

Original publication

DOI

10.1016/j.chembiol.2008.01.009

Type

Journal article

Journal

Chem Biol

Publication Date

03/2008

Volume

15

Pages

246 - 253

Keywords

Arabidopsis, Cytochromes c, Heat-Shock Proteins, Small, Multiprotein Complexes, Nanotechnology, Plant Proteins, Protein Structure, Quaternary, Robotics, Spectrometry, Mass, Electrospray Ionization, Trypsin