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Toll-like receptors (TLRs) mediate responses to pathogen-associated molecules as part of the vertebrate innate immune response to infection. Receptor dimerization is coupled to downstream signal transduction by the recruitment of a post-receptor complex containing the adaptor protein MyD88 and the IRAK protein kinases. In this work, we show that the death domains of human MyD88 and IRAK-4 assemble into closed complexes having unusual stoichiometries of 7:4 and 8:4, the Myddosome. Formation of the Myddosome is likely to be a key event for TLR4 signaling in vivo as we show here that pathway activation requires that the receptors cluster into lipid rafts. Taken together, these findings indicate that TLR activation causes the formation of a highly oligomeric signaling platform analogous to the death-inducing signaling complex of the Fas receptor pathway.

Original publication

DOI

10.1074/jbc.M109.022392

Type

Journal article

Journal

J Biol Chem

Publication Date

11/09/2009

Volume

284

Pages

25404 - 25411

Keywords

Cell Line, Cross-Linking Reagents, Gene Expression Regulation, Humans, Interleukin-1 Receptor-Associated Kinases, Mass Spectrometry, Membrane Microdomains, Models, Biological, Myeloid Differentiation Factor 88, Protein Structure, Tertiary, Scattering, Radiation, Signal Transduction, Ultracentrifugation, X-Rays, fas Receptor