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Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

Original publication




Journal article


Opt Lett

Publication Date





3154 - 3156


Air, Animals, Cytoplasm, Developmental Biology, Embryo, Mammalian, Equipment Design, Glass, Image Processing, Computer-Assisted, Mice, Microscopy, Confocal, Models, Statistical, Optics and Photonics, Refractometry, Time Factors