Developing a Crispr/Cas9-mediated system for regulation of local chromatin configuration
Epigenetic reprogramming underlies cell fate changes in development and regeneration. However appropriate tools to study these changes are currently lacking. We have developed a strategy to change local chromatin structure in a specific manner based on the Crispr/Cas9 system. Specifically, we generated a fusion protein of the enzymatic domain of the H3K9me2 methyl transferase G9a with an endonuclease mutant version of the Cas9 protein for lentiviral delivery. When combined with gene-specific guide RNA, this will result in targeted, local chromatin modification.