Developing a Crispr/Cas9-mediated system for regulation of local chromatin configuration

Cambridge

£23,678

2015

Epigenetic reprogramming underlies cell fate changes in development and regeneration. However appropriate tools to study these changes are currently lacking. We have developed a strategy to change local chromatin structure in a specific manner based on the Crispr/Cas9 system. Specifically, we generated a fusion protein of the enzymatic domain of the H3K9me2 methyl transferase G9a with an endonuclease mutant version of the Cas9 protein for lentiviral delivery. When combined with gene-specific guide RNA, this will result in targeted, local chromatin modification.